Paper Information

Title: 

THE EFFECT OF VERAPAMIL ON ANTI-SEIZURE PROPERTIES OF CANNABINOID CB1 RECEPTOR AGONIST IN PENTYLENETETRAZOLE MODEL OF SEIZURE IN MICE

Type: POSTER
Author(s): ESLAHKAR S.*,GHEYSARZADEH N.,NADERI NIMA,MOTAMEDI F.
 
 *SCHOOL OF MEDICINE, SHAHED UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Previous studies revealed the anti-seizure effect of cannabinoids in different types of seizure models in laboratory animals. Cannabinoids play a role in setting the threshold of excitability of neurons through the presynaptic CB1 receptor. Calcium channels, both in the cell membrane and inside the cytoplasm of neurons, are affected by cannabinoid receptors. In this research, the effects of an L-type calcium channel blocker (verapamil) and a cannabinoid CB1 receptor agonist (ACEA) on pentylenetetrazole-induced seizure model in mice were studied. Verapamil and ACEA were injected intraperitoneally alone or in combination 30 min before the seizure test. In order to take the seizure test, a pentylenetetrazole 1% solution was used and injected into the tail vein of animal using infusion pump with fixed speed of 0.25 ml/min. Generalized clonic seizure of animal with loss of righting reflex was considered as the endpoint of experiment. Injection of ACEA (2mg/kg) leads to significant increase of PTZ dose required for seizure induction of mice compared with control group. Injection of various doses of verapamil did not produce significant change of PTZ dose needed to induce seizure compared with control group. The simultaneous injection of different doses of verapamil along with ACEA (2mg/kg) did not produce any significant changes in anti-seizure effect of ACEA. The results showed that inhibition of L-type calcium channel by verapamil did not affect the anti-seizure properties of cannabinoid CB1 receptor agonist.

 
Keyword(s): VERAPAMIL, ACEA, CANNABINOID, L-TYPE CALCIUM CHANNEL BLOCKER, SEIZURE, PENTYLENETETRAZOLE
 
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