Paper Information

Title: 

STIMULATORY EFFECT OF SAFRANAL ON β2-ADRENOPCEPTORS OF GUINEA PIG TRACHEAL CHAINS

Type: POSTER
Author(s): AHMADZADEH HOSSEIN*,BOSKABADI M.H.,NEMATI H.A.
 
 *ZAHEDAN MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Background: As indicated in ancient Iranian medical books Crocus sativus has therapeutic effects on respiratory diseases. The relaxant effect of this plant has also been observed on smooth muscles including guinea pig’s tracheal chain in previous studies. To study the mechanism(s) of the relaxant effect of this plant, in this study the stimulatory effect of safranal, main constituent of Crocus sativus, on b-adrenoceptors was examined on tracheal chains of guinea pigs
Methods: The
b2-Adrenergic stimulatory effect of two concentrations of safranal (1.25 and 2.5 μg), 10 Nm propranolol, and saline were tested by performing the cumulative concentrationresponse curves of isoprenaline induced relaxation of precontracted isolated guinea pig tracheal chains with 10μM metacholine in two different conditions including: non incubated (group 1, n=9) and incubated with 1 μM chlorpheniramine (group 2, n=6). The effective concentration of isoprenaline, causing 50% of maximum response (EC50), maximum response and the slope of isoprenaline curves obtained in the presence of safranal, and propranolol were compared with those of saline. The shift in concentration response curves due to propranolol and safranal was calculated as (CR-1) by: (EC50 obtained in the presence of active substances/EC50 obtained in the presence of saline) -1.
Results: The results showed clear leftward shifts in isoprenaline curves obtained in the presence of only higher concentration of safranal in group 2 compared with that of saline in group 2. The EC50 obtained in the presence of both concentrations of safranal in group 1 was significantly lower than that of saline (p<0.05 for all cases). The maximum responses obtained in the presence of both concentrations of safranal in group 1 was significantly lower than that of saline (p<0.005 for all cases). However, in group 2, there was no significant difference between maximum responses obtained in the presence of two concentrations of safranal compared to that of saline. All values of (CR-1) obtained in the presence of higher concentration of safranal in group 2 were negative while those obtained in the presence of propranolol were positive and there were significant differences in this value between propranolol and those obtained in the presence of safranal (p<0.05 to p<0.001).
Conclusion: The results of this study indicated a relatively potent stimulatory effect of safranal on
b2-adrenoceptors. A possible inhibitory effect of safranal on histamine (H1) receptors also suggested.

 
Keyword(s): SAFRANAL, BRONCHODILATORY, GUINEA PIG, TRACHEA
 
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