Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

MODULATION OF OXIDATIVE STRESS STATE OF NEUROBLASTOMA SK-N-MC CELLS BY Aβ1-42 FIBRIL

Type: POSTER
Author(s): SHEYKH ALISHAHI HAMED,YAZDANPARAST RAZIEH*
 
 *INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

1-42 is the main constituent of senile plaques characterized in Alzheimer's disease (AD). It is repeatedly demonstrated that Aβ aggregation is heavily involved in the pathogenesis of AD. On the other hand, it has been shown that oxidative stress is closely linked to Aβ cytotoxicity. In this study, to prepare the fibrillar Aβ1-42, the peptide was dissolved in PBS and kept at 37°C for 10 days. Treatment of SK-N-MC cells with Aβ1-42 (10 μM) led to nearly 50% reduction in cell viability determined by MTT assay. A1-42-induced cell death was determined by using ethidium bromide/ acridine orange staining. Moreover, the intracellular level of ROS in Aβ-treated (10 μM) cells. Aβ1-42 has caused an increase in the extent of intracellular peroxide radicals by 90%, relative to untreated control cells. Furthermore, treatment of SK-N-MC cells with 10 μM Aβ1-42 increased the activity of superoxide dismutase enzyme by about 70 % and decreased the activity of catalase by 36%, respectively relative to untreated control cells. Numerous studies suggest that amyloid beta aggregates with different conformations might have dissimilar effects on the different neuroblastoma cell lines. Thus, collecting and comparing those data might be useful to explore, in more details, mechanisms involved in Aβ cytotoxicity.

 
Keyword(s): ALZHEIMER'S DISEASE, CATALASE, ROS, OXIDATIVE STRESS,SK-NMC CELLS, SUPEROXIDE DISMUTASE
 
 
Yearly Visit 35   tarjomyar
 
Latest on Blog
Enter SID Blog