Paper Information

Title: 

POSSIBLE INVOLVEMENT OF ENDOGENOUS NEURAL STEM CELLS IN REMYELINATION PROCESS FOLLOWING LOCAL INJECTION OF LYSOLECITHIN IIN ADULT RATS HIPPOCAMPUS

Type: POSTER
Author(s): AZIN MAHDIEH,JAVAN MOHAMMAD,MIRNAJAFIZADEH S.J.,SATARIAN L.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). Demyelination may be followed by remyelination, a spontaneous regenerative response. Remyelination represents one of the most compelling examples of adult multipotent progenitor cells contributing in regeneration of the injured CNS. In MS, myelin-forming oligodendrocytes are the targets of inflammatory and immune attacks. Myelin basic protein (MBP) contributes to the formation and compaction of myelin sheath. Oligodendrocyte precursor cells (OPCs) initially express olig2 transcription factor. Nestin has been utilized as a biological marker to identify neural stem cells (NSCs). The adult hippocampus is extremely vulnerable to various insults such as MS. The purpose of this study is to investigate changes in the expression of MBP, Olig2 and Nestin in the context of lysolecithin induced demyelination in hippocampus. Adult male Sprague-Dawley rats weighing 280-320 g underwent stereotaxical injection of saline or lysolecithin into the right hippocampus. Gene expression for MBP, Olig2 and Nestin was evaluated in intact and saline treated- and lysolecithin-treated rats on days 2, 7, 14 and 28 post lesions. There was no significant difference in MBP, Olig2 and Nestin gene expression between intact and saline groups. Decreased expression of MBP and increased expression of Olig2 and Nestin in 2 and 7 days post lesion in lysolecithin group were observed. After 28 days, MBP gene expression increased and Olig2 and Nestin gene expression decreased toward control level. Our data showed the presence of demyelination and remyelination processes following lysolecithin injection into the hippocampus. Remyelination process seemed to be mediated by the contribution of NSCs and OPCs.

 
Keyword(s): DEMYELINATION, REMYELINATION, LYSOLECITHIN, HIPPOCAMPUS, MBP, OLIG2, NESTIN, NEURAL STEM CELLS, OLIGODENDROCYTE PRECURSOR CELLS, RAT
 
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