Paper Information

Title: 

THE EFFECTS OF SEVERAL GROWTH FACTORS ON DOPAMINERGIC DIFFERENTIATION OF AMNIOTIC EPITHELIAL CELLS

Type: POSTER
Author(s): NIKNEZHAD HASAN*,PEYROVI HABIB ELAH,AHMADIANI A.A.H.,GHANAVI J.,JORJANI M.
 
 *NANOMEDICINE AND TISSUE ENGINEERING RESEARCH CENTER, SHAHEED BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The differentiation of dopaminergic neurons from embryonic stem cells is influenced by several factors. Amniotic epithelial cells (AECs) share many of the same characteristics as embryonic stem cells, and therefore those factors may similarly affect the derivation of dopaminergic neurons from AECs. In this study, we examined the differentiation of dopaminergic neurons in vitro from AECs using the expression of several markers including β-tubulin III, TH, DAT and DβH following AECs treatment with retinoic acid (RA), basic fibroblast growth factor (bFGF), sonic hedgehog (Shh) and FGF8. We also studied whether blocking bone morphogenetic protein (BMP) signaling using its antagonist, noggin, affects the derivation of neural cells from AECs. Analysis of AECs derived dopaminergic neurons was performed at the β-tubulin III, TH and DAT expression levels by immunocitochemistry. All cultures treated with noggin showed the higher levels of β-tubulin III expression than noggin free cultures. Combined treatment with bFGF and RA showed the highest level of β-tubulin III in all treatment groups with or without noggin. bFGF withdrawal did not promote expression of β-tubulin III, while its maintenance increased the expression of the neural marker. Combination of Shh and FGF8 showed the higher level of TH in comparison to control group or these factors alone. Moreover, Shh is more effective than FGF8on DAT expression in comparison to expression of DβH. These results show the capability of AECs to express dopaminergic neural markers and this ability is affected by some factors including noggin, bFGF, RA, Shh and FGF8.

 
Keyword(s): AMNIOTIC EPITHELIAL CELLS, DOPAMINERGIC DIFFERENTIATION, NOGGIN, RETINOIC ACID, BFGF, SHH, FGF8
 
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