Paper Information

Title:  CYTOTOXIC EFFECT OF HONEY ON HUMAN BREAST CARCINOMA CELL LINE (MCF-7) COMPARED WITH NORMAL HUMAN FETAL LUNG FIBROBLAST CELL LINE (MRC-5) IN VITRO
Type: POSTER
Author(s): DAVARI B.S.,SAMARGHANDIAN S.,PARSAEI H.,TAVAKOL AFSHARI J.,TAYARANI Z.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Honey is a beneficial and common house hold product with many medicinal uses described in traditional medicine. The results of the studies prove that it helpful in wound healing, dental heath and diabetes. It also has a potential therapeutic role in the treatment of diseases due to its antioxidant, antimicrobial properties and higher amount of phenolic and phytochemical constituents. The aim of this study was to investigate the effect of honey on MCF-7 cell line (human breast carcinoma) and MRC-5 cell line as control. Both cell lines were treated with (0, 0.1%, 1%, 10%, 20%) of honey in culture medium for 24 and 48 hours. Then cells were studied under light inverted microscope for morphological features such as shape granulation and anchorage independency, and at the same time antiproliferative properties of honey were determined by MTT colorimetric assay. The results showed that some concentrations of honey (10% and 20%) decreased cell viability in malignant cells compared to MRC-5. Morphology of MCF7 cells treated with honey confirmed MTT results. Lower concentrations of honey (0.1% and 1%) didn't show morphological changes.Therefore, it seems, honey with the ability to inhibit breast cancer-derived cell line, probably has therapeutic effect on breast cancer cell line.

 
Keyword(s): HONEY, MRC-5, MCF-7, CYTOTOXICITY, MTT
 
Yearly Visit 7  
 
Latest on Blog
Enter SID Blog