Paper Information

Title: 

CAUDAL PORTION OF OPTIC CHIASM UNDERLIES MORE REMYELINATION THAN ROSTRAL PART FOLLOWING LYSOLECITHININDUCED EXPERIMENTAL DEMYELINATION IN RAT

Type: POSTER
Author(s): MOZAFARI SABAH*,JAVAN M.,TIRAHI T.
 
 *DEPARTMENT OF PHYSIOLOGY, FACULTY OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Multiple Sclerosis (MS) is the main demyelinating disease in young adults. Toxic models of demyelination are used for assessment of endogenous remyelination capacity of adult CNS. Demyelination affects the optic apparatus in more than 70% of individuals suffering from MS. Lesions in vicinity of lateral ventricles undergo more remyelination because of the existence of endogenous multipotent migrating remyelinating cells in subventricular zone. The 3rd ventricle which is placed in vicinity of optic chiasm contains multipotent cells. In this study, remyelination pattern in the longitude of experimentally demyelinated optic chiasm and subsequent proximal nerves was investigated. Local stereotaxic microinjection of lysolecithin 1% (2 μl) was preformed into female rat optic chiasm (bregma: -280). Histological assessment was performed on coronal 5 micron serial sections with 40 micron intervals using specific myelin staining at 2, 7, 14 and 28 days post lesion (dpl) from +120 to -680 μm related to bregma. The extent of demyelination was compared for points of +40 and -600 μm in the mentioned days. Lysolecithin can diffuse in an area with 800-1500 μm in length. In point of bregma -600, the extent of demyelination was increased on 2 dpl and was significant on days 7 (p<0.01), 14 (p<0.001) and 28 (p<0.05) dpl statistically, however, it was significantly decreased on day 28 compared to day 14 post lesion (p<0.05). In point +40 the extent of demyelination was increased on days 7, 14 and 28 post lesion; nevertheless, on day 28 in comparison with 14 dpl, it decreased insignificantly. Lesion undergoes remyelination by time; however, this reconstruction in caudal part of chiasm was more significant than that in rostral part. Less distance of caudal part from the 3rd ventricle which is reported to contain multipotent stem cells in adult may be the cause of this observation. Our findings may support the idea of the 3rd ventricle stem cells participation in myelin repair.

 
Keyword(s): MS, LOCAL DEMYELINATION, LYSOLECITHIN, OPTIC CHIASM AND NERVES, REMYELINATION, RAT
 
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