Paper Information

Title:  CHANGES BRAIN EDEMA AND TGF-β LEVELS IN COADMINISTRATION OF ESTROGEN AND PROGESTERONE IN FEMALE RATS WITH DIFFUSE TRAUMATIC BRAIN INJURY.
Type: POSTER
Author(s): SOLTANI ZAHRA*,KHAKSARI MOHAMMAD,SHAHROKHI NADER,MOSHTAGHI GH.R.
 
 *NEUROSIENCE AND PHSIOLOGY RESEARCH CENTERS, KERMAN UNIVERSITY OF MEDICAL SCIENCES, KERMAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Introduction and Objectives: Our previous study demonstrated that alone administration of estrogen or progesterone results in reduction of brain edema and blood-brain barrier permeability following diffuse TBI. Role of TGF-β in neurologic diseases has been demonstrated. In this study we investigated effect of estrogen and progesterone in combination on brain edema and TGF-β levels following traumatic brain injury (TBI) in female rats.
Materials And Methods: In this interventional-experimental study randomly assigned in 8 groups (7 groups OVX and 1 group intact) that any group was consisted of two subgroups as follows: 1: control,2: sham,3: ovarectomized trauma (TBI+OVX), 4: vehicle, 5: physiologic dose of estrogen+physiologic dose of progesterone (E1+P1), 6: physiologic dose of estrogen+pharmacologic dose of progesterone (E1+P2), 7: pharmacologic dose of estrogen+physiologic dose of progesterone (E2+P1), 8: pharmacologic dose of estrogen+pharmacologic dose of progesterone (E2+P2). Rats exposed diffuse traumatic brain injury through marmarou model. Hormones were injected i.p a half hour after diffuse traumatic brain injury. Brain edema and blood-brain barrier permeability and brain TGF-β levels were measured in this animals at 24 hours after of brain injury.
Results: Brain TGF-β levels showed a significant increase in TBI group comparing to sham group (P<0.01). Also brain TGF-β levels found a significant increase in vehicle group comparing to E1+P1 and E1+P2 groups (P<0.001). Brain water content demonstrated a significant increase in TBI group compared with control and sham groups (P<0.001).This content also showed a significant decrease in E1+P1 and E2+P2 groups compared with vehicle group (P<0.01). Brain Evans blue showed a significant increase in TBI group comparing to control and sham groups (P<0.01). This content demonstrated a significant decrease in E2+P1 group compared with sham and vehicle groups (P<0.05).
Discussion and Conclusion: combined administration of estrogen and progesterone have beneficial effect in both the reduction of brain edema depends on what dose of estrogen is administrated with progesterone. Administration of estrogen physiologic dose in combination decreased Brain TGF-β levels. Changes of TGF-β in combined administration of sex hormones may be one mechanism by which these sex steroids hormones reduce cerebral edema.

 
Keyword(s): DIFFUSE TRAUMATIC BRAIN INJURY, ESTROGEN, PROGESTERONE, TGF-β, BLOOD-BRAIN BARRIER PERMEABILITY, WATER CONTENT
 
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