Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

A SUITABLE VISIBLE SPECTROPHOTOMETRIC METHOD FOR EVALUATING OF EXPOSURE TO CYANOGEN COMPOUNDS

Type: SPEECH
Author(s): HOSSEINI ROUH ELAH*,YOUSEFIAN S.,ELMI RANKOHI K.
 
 *DEPARTMENT OF TOXICOLOGY AND PHARMACOLOGY, FACULTY OF PHARMACY, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

One of the most important side effects in patients with chronic exposure to cyanogen compounds is endemic goiter. Because the resulted compound, thiocyanate, compete to iodine for absorption by thyroid gland. Therefore, we developed a simple and reliable visible spectrophotometric method for assay of thiocyanate in urine samples. In this method, the thiocyanate of samples is oxidized in acidic medium for liberation of HCN. The liberated HCN reacts with a picrate on filtration paper in a closed vial. The colored complex is extracted into water and then is assayed its absorbance at 500 nm. Our results showed that there are no any interferences with urine samples containing less than 7 g/L protein, and up to 40 mg/L glucose, 20 mg/L NaCl and 100 mg/L urea. The standard curve was linear up to 100 mg/L of thiocyanate. The recovery, within-run and between-run precisions were 99.95%, 4.44% and 5.82%, respectively. The detection limit of the present method was 0.45 mg/L. The suitable oxidizing agent was potassium permanganate. The results indicated that thiocyanate in urine was stable at least for 2 weeks at 4 ºC and for one month at -20 ºC. Finally, this method is suitable and reliable for monitoring patients, especially with endemic goiter exposed to natural and synthetic cyanogen compounds.

 
Keyword(s): 
 
Yearly Visit 48   tarjomyar
 
Latest on Blog
Enter SID Blog