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Paper Information

Title: 

PROTECTIVE ROLE OF ESTRADIOL IN RABBIT SPINAL CORD ISCHEMIA: FOCUS ON CYCLOOXYGENASE-1, CYCLOOXYGENASE-2, HEAT SHOCK PROTEIN 70 AND CLEAVED CASPASE-3

Type: POSTER
Author(s): KHALAJ L.*,PEYROVI HABIB ELAH,KHODAGHOLI F.,ABDI AZADEH,DARGAHI L.,MOHAGHEGHI F.,AHMADIAN ABOU ALHASAN
 
 *NEUROSCIENCE RESEARCH CENTER, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Postoperative neurologic deficits such as paraplegia or paraparesis are the most devastating complications after thoracoabdominal aortic aneurysm repair due to inadequate blood supply to lumbar spinal cord during these operations. Despite demonstrated neuroprotective effects of estradiol, its protective efficacy against spinal cord ischemia-reperfusion and underlying mechanisms are not yet elucidated. We investigated the efficacy of estradiol in attenuating spinal cord ischemia-reperfusion-induced pathology through neurological, histopathological, biochemical (Malondialdehyde and GSH level as well as Catalase and Superoxide Dismutase activities were measured) and western blot assessments. 25 New Zealand White rabbits were divided into experimental groups as follows: Sham (n=5), Ischemic Control (n=10) which received sesame oil as vehicle, 30 min before ischemia (ischemia was induced on lumbar spinal cord through clamping infrarenal abdominal aorta for 18 min) and Estradiol (n=10) which received single i.m. injection of 17-β-estradiol cypionate, 30 min before ischemia. Results showed that administration of estradiol before induction of spinal cord ischemia improved functional outcome and prevented the worsening pattern of neurological function observed in ischemic control group, over 48 hours of this study. Near to normal histopathological outcome and oxidative stress status of lumbar spinal cords, further confirmed neuroprotective effects of estradiol. Interestingly, significant cyclooxygenase-1 induction in spinal cord tissue of these animals seemed to be protective. Estradiol also reduced spinal cord cyclooxygenase-2, Heat shock protein 70 and cleaved caspase-3 in this ischemic context. Taken together, our results revealed that acute estradiol can provide neuroprotection against spinal cord ischemiareperfusion and suggest that induction of cyclooxygenase-1 and reduction of oxidative stress, cyclooxygenase-2, heat shock protein 70 and cleaved caspase-3, all may be involved in the protection mechanism. Estradiol can be considered as a potential candidate to protect against spinal cord ischemia-reperfusion-induced paraplegia resulting from thoracoabdominal aortic aneurysm repairs.

 
Keyword(s): ESTRADIOL, SPINAL CORD ISCHEMIA-REPERFUSION, CYCLOOXYGENASE-1, CYCLOOXYGENASE-2, HEAT SHOCK PROTEIN 70, CLEAVED CASPASE-3
 
 
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