Paper Information

Title: 

APOPTOSIS INDUCTION IN MOTOR NEURONS OF CULTURED SPINAL CORD SLICES FROM ADULT MOUSE

Type: POSTER
Author(s): NASIMI PARVA*,MOUMENI HAMID REZA,SOLEYMANI MEHRANJANI M.,ABNOUSI M.H.
 
 *BIOLOGY DEPARTMENT, FACULTY OF SCIENCE, ARAK UNIVERSITY, ARAK, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The viability of central nervous system slices e.g. spinal cord from adult mammals is especially difficult to maintain in culture and rapidly deteriorates in vitro. However, such slice cultures can be a good in vitro model for studying cell death and the mechanisms involved. Since motor neuron death is a critical phenomenon in spinal cord injuries, the present study was organized to investigate cell death in motor neurons of adult mouse spinal cord slices. Thoracic region of spinal cord from adult Balb/c mice was cut into 400μm-thick slices using a tissue chopper and the slices were cultured in a CO2 incubator at 37ºC for 3, 6, 12 and 24 h. Freshly prepared and the cultured slices were fixed in Stefanini's fixative and sectioned using a cryostat. To study motor neuron death, fluorescent staining (Hoechst33342 and propidium iodide), TUNEL method and agarose gel electrophoresis were used. At early time point of slice culture, motor neurons not only displayed morphological features of apoptosis including cell shrinkage as well as nuclear and chromatin condensationbut also were TUNEL positive. In addition, slices cultured for 24 h showed nucleosomal DNA fragmentation (DNA ladder). In conclusion, the results show the occurrence of apoptosis in motor neuron cultured spinal cord slices from adult mouse.

 
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