Paper Information

Title: 

EVALUATION OF LOVASTATIN AND α-TOCOPHEROL EFFECT ON INDUCED APOPTOSIS IN HUMAN COLORECTAL CARCINOMA CELL LINE

Type: POSTER
Author(s): ARAST Y.,REZAEI MOHSEN,GALEHDARI HAMID
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Apoptosis plays a significant role in tumor development and it has been hypothesized that lack/failure of apoptosis leads to the development of tumors, such as colon tumors. Thus, induction of apoptosis in tumor cells is an effective approach to the regulation of tumor growth. It has been shown that various chemopreventive agents induce apoptosis and inhibit tumor growth. Identification of agents or combinations of agents that induce tumor cell apoptosis help in the development of novel agents for colon cancer treatment. This study has been designed to assess the effectiveness of lovastatin, (a 3-hydroxy-3-methyl glutaryl-CoA reductase inhibitor), and alpha tocopherol (a micronutrient), individually or in combination on the induction of apoptosis in human HT29 colon cancer cells. HT29 cells were exposed to various doses of lovastatin and/or alpha tocopherol followed by DNA fragmentation method. Pretreatment with lovastatin (10-30 micromolar) induce apoptosis in HT29 cells at concentrations that have been studied. The same results were obtained with alpha tocopherol (5-20 micromolar). In combination, results obtained in this investigation showed significant results. In tumor cells that have been exposed to 10 micromolar lovastatin plus 5 and 10 micromolar tocopherol, cell death was not shown.According to the various mode of action and pleiotropic effects of statins in altering cell function and death; we postulate that the concentration of lovastatin is one of the most important parameter for selecting the type of action. These findings also support the hypothesis that HMGCoA-R and tocopheroles may play a role in regulation of apoptosis and provide effective strategies for the prevention of colon cancer.

 
Keyword(s): COLORECTAL CANCER, CELL DEATH, LOVASTATIN, TOCOPHEROL
 
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