Paper Information

Title: 

EFFECTS OF SODIUM ARSENITE ON INDUCTION OF APOPTOSIS IN RATS BONE MARROW MESENCHYMAL STEM CELLS

Type: POSTER
Author(s): ABNOUSI M.H.,SOLEYMANI MEHRANJANI M.,MOUMENI HAMID REZA,SHOJAEIFAR E.,BARATI MOZHGAN
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Sodium arsenite (SA) is used as medicin, pestisides as well as in many other industries, which finally can cause the toxicity of human and other animals. Our previous investigations show that the treatment of the rats Bone Marrow Mesenchymal Stem Cells (rBMMSCs) with SA cause reduction of their viability. The aim of the present study was to investigate the mechanism of the cell death due to SA exposure. The cells after 3rd passage was treated with 0.1μM SA for a period of 36 hrs, then using TUNEL, caspase and comet assay as well as morphological staining using acr……ange and ……t 33342, the cell death was investigated. The results showed that the SA treated cells, were TUNEL positive and activated caspase 3 enzym……t……ir cytoplasm was also visualized. In addition morphology of the cells showed chromosomal condensation as well as cytoplasm shrinkage. Comet assay also reviled that the DNA of the treated cells was broken. In conclusion, SA treatment caused caspase dependent program cell death in rat’s bone marrow mesenchymal stem cells.

 
Keyword(s): SODIUM ARSENITE, APOPTOSIS, CASPASE 3, MESENCHYMAL STEM CELLS, COMET ASSAY
 
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