Paper Information

Title:  THE INDUCTION OF APOPTOSIS AND AUTHOPHAGY IN RATS BONE MARROW MESENCHYMAL STEM CELLS FOLLOWING IN VITRO TREATMENT WITH P-NONYLPHENOL
Type: POSTER
Author(s): SOLEYMANI MEHRANJANI M.,ABNOUSI M.H.,MOUMENI HAMID REZA,BARATI MOZHGAN,SHOJAEIFAR E.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

p-Nonylphenol (p-NP) as a non ionic surfactant is used in many industries such as pesticides, cosmetics, detergents and so on. Our previous study showed that the p-NP causes reduction in viability of the rats Bone Marrow Mesenchymal Stem Cells (rBMMSCs). The aim of the present study was to investigate the mechanism of the cell death due to p-NP exposure. The cells after 3rd passage was treated with 100μM p-NP for a period of 36 hrs, then using TUNEL, caspase and comet assay as well as acridine orange and monodensylcadaverin (MDC) staining the cell death was studied. The results of this study showed that the p-NP treated cells, were TUNEL positive and activated caspase 3 enzymes in their cytoplasm was also visualized. In addition, staining the cells with acridine orange reviled that the cytoplasms of the treated cells contain numerous vesicles, which the staining of the cells with the help of MDC showed presence of the double layer autophagic vacuoles. In conclusion, p-NP treatment induced cell death due to caspase dependent apoptosis as well as autophagy.

 
Keyword(s): P-NONYLPHENOL, APOPTOSIS, AUTOPHAGY, MONODENSYLCADAVERIN, CASPASE 3
 
Yearly Visit 7  
 
Latest on Blog
Enter SID Blog