Paper Information

Title: 

INTERACTION BETWEEN ACUTE MORPHINE ADMINISTRATION AND CANNABINOID SYSTEM ON FAST INHIBITORY POST SYNAPTIC TRANSMISSION OF SUPRAOPTIC NUCLEUS NEURONS IN RAT

Type: POSTER
Author(s): YOUSEFPOUR MITRA*,NADERI NIMA,MOTAMEDI F.
 
 *RESEARCH CENTER OF NEUROSCIENCE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The magnocellular neurons (MCN) of the Supraoptic Nucleus (SON) play a crucial hormonal role due to two peptides they synthesize, Oxytocin and Vasopressin. The activity of magnocellular neurons is regulated by both intrinsic ionic conductance and a variety of excitatory and inhibitory inputs. A wide variety of transmitters can modulate the efficacy of afferent transmission onto these cells. Opioids and Cannabinoids are two important transmitters that have been shown to influence MCN activity. It is now well-established that there is an interaction between opioids and cannabinoids in some areas in the central nervous system. But, no data are available on such possible interaction in the SON. Therefore the aim of the present study was to investigate a possible interaction between morphine and cannabinoid system on inhibitory post synaptic currents (IPSCs) in rat SON neurons using the whole cell patch clamp technique. Male Wistar rats (3– 4 weeks old) were used for this study. Animals were anesthetized with isoflurane and decapitated. The brain was quickly removed and coronal slices (250μm) were cut with a vibratome. Membrane currents were recorded using an Axopatch-200B. The results showed that morphine (25μM) decreased IPSCs but the cannabinoid agonist (URB597, 100nM) increased IPSCs in MCN. However, the inhibitory effect of morphine on magnocellular neurons’ IPSCs was diminished in presence of URB597. In conclusion, it seems that cannabinoids can modulate effects of morphine on post synaptic currents in the SON.

 
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