Paper Information

Title: 

EFFECTS OF L-CARNITINE AND α-TOCOPHEROL ON ACUTE URETERAL OBSTRUCTION-INDUCED DISTURBANCES OF RENAL OXIDATIVE BALANCE, ENERGY METABOLISM, AND ACID EXCRETION IN ANAESTHETIZED RATS

Type: SPEECH
Author(s): CHANGIZI ASHTIANI SAEID*,SHID MOUSAVI SEYED MOSTAFA,HOSSEINKHANI S.,SHIRAZI MAHDI
 
 *DEPARTMENT OF PHYSIOLOGY, SCHOOL OF MEDICINE, ARAK UNIVERSITY OF MEDICAL SCIENCES, ARAK, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Ureteral obstruction has been shown to induce renal oxidative stress, suppressed energy metabolism and defected acid excretion. This study was aimed to examine the improving effects of L-carnitine, a facilitating cofactor for mitochondrial oxidation of fatty-acids as well as a scavenger of free-radicals, and a =-tocopherol as the most potent antioxidant on these renal disorders at early hours following release of unilateral ureteral obstruction. The left ureter was ligated in lightly anaesthetised rats, in which L-carnitine, a-tocopherol, or their vehicles (normal saline and olive oil, respectively) were injected (i.p.) in four different groups. Each rat was re-anesthetized and cannulated, and ureteral ligation was released at exactly 24 h after UUO-induction. A 30-min clearance period was performed to separately collect urine from both kidneys. The collected urine and arterial blood samples were given to pH-gas analyzer and autoanalyzer, and preserved kidneys were assessed to measure malondialdehyde (MDA), ATP and ADP levels. There were also sham and control groups (n= 8-10 in each group). In the post-obstructed kidney of vehicle-treated groups with respect to the equivalent kidney of sham group, there were increases (P<0.05-0.001) in MDA, ADP, urinary pH, absolute and fractional bicarbonate excretions, but decreases (P<0.01-0.001) in ATP, ATP/ADP, and urinary Pco2. a-tocopherol could normalize MDA level but did not affect the altered amounts of energy metabolic indices and acid-base excretions, while L-carnitine improved all of them except of decreased urinary Pco2. Therefore, increased bicarbonate-excretion in post-obstructed kidney was due to defected acid-secretion at collecting duct, which was not related to ureteral obstruction-induced renal oxidative stress and suppressed energy metabolism.

 
Keyword(s): RENAL OXIDATIVE STRESS, URETERAL OBSTRUCTION, L-CARNITINE, α-TOCOPHEROL
 
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