Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

PHENYLEPHRINE PRODUCES EARLY AND LATE PHARMACOLOGICAL PRECONDITIONING VIA OPENING OF MITOCHONDRIAL KATP CHANNEL IN THE ISOLATED RAT HEART

Type: SPEECH
Author(s): NADERI ROYA*,FAGHIHI MAHDIEH,IMANI A.R.,HOUSHMAND F.
 
 *
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

We assume that phenylephrine produces late pharmacological preconditioning through opening of the mitochondrial kATP channel. To test this hypothesis; rat hearts were isolated and perfused with krebs buffer in langendorff mode and subjected to 30 min regional ischemia followed by 60 min of reperfusion. In this study, phenylephrine as a selective a1-adrenoceptor agonist and 5-hydroxydecanoate (5HD) as a specific blocker of the mitochondrial kATP channel were used. Rats were randomly divided into six groups (n=6): (i) control: surgical procedure was performed with no ischemia/reperfusion, (ii) ischemia/reperfusion: hearts underwent regional ischemia/reperfusion, (iii) phenylephrine early preconditioning: phenylephrine (50μm) was perfused for 5 min prior to ischemia/reperfusion, (iv) phenylephrine late preconditioning: rats were treated with phenylephrine (10mg/kg, i.p) 24 h prior to ischemia/reperfusion, (v) phenylephrine early preconditioning-5HD: 5HD (100μm) was perfused for 5 min prior to phenylephrine as seen in group iii , (vi) phenylephrine late preconditioning-5HD: 5HD (100μm) was perfused for 5 min prior to ischemia/reperfusion as seen in group iv. Compared to ischemia/reperfusion group, phenylephrine in early and late phases decreased myocardial infarct size (% of ischemia zone), redused CKMB (creatine kinase-mb) release in the coronary effluent and improved cardiac function. Pretreatment with 5HD abolished phenylephrine-induced cardioprotection in early and late phases. We conclude that phenylephrine-induced late cardioprotection was abolished by adminstration of 5HD in the isolated rat hearts.

 
Keyword(s): PHENYLEPHRINE, 5-HYDROXYDECANOATE, CARDIOPROTECTION, ISCHEMIA/REPERFUSION, MITOCHONDRIAL KATP CHANNEL
 
 
Yearly Visit 35   tarjomyar
 
Latest on Blog
Enter SID Blog