Paper Information

Title: 

CO-ADMINISTRATION OF VITAMIN E AND D3 POTENTIATES MYELIN REPAIR AND DECREASES DEMYELINATION FOLLOWING INTRA-HIPPOCAMPAL INJECTION OF ETHIDIUM BROMIDE IN RATS

Type: SPEECH
Author(s): GOUDARZVAND M.*,JAVAN M.,MIRNAJAFIZADEH S.J.,ALTARIHI T.,MOZAFARI S.
 
 *DEPARTMENT OF PHYSIOLOGY, SCHOOL OF MEDICAL SCIENCES, TARBIAT MODARES UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Multple Sclerosis (MS) is an autoimmune and inflammatory demyelinating disease. Antioxidant vitamins D and E are recommended for treatment of patients suffering from neurodegenerative disease like multiple sclerosis (MS), while their mechanism of action is not well-understood. Here, we studied the effect of co-administration of these vitamins on demyelination, cell death and remyelination of rat hippocampus following local ethidium bromide (EB) injection. EB was directly injected into the rat’s Dentate Gyrus. Animals received 100 mg/kg vitamin E and 5μg/kg of vitamin D3 for 7 days. The extent of demyelination, myelin staining intensity, and the expression of myelin basic protein (MBP) and Caspase-3 were studied using histological and immunoblotting verification. There was no significant difference between intact, saline- and vehicle-treated groups in Caspase 3 and MBP expression. While the administration of EB alone increased the expression of caspase-3 and decreased the expression of MBP, co-administration of vitamins E and D3 decreased the expression of caspase-3 (p<0.001), and also increased MBP in EB treated animals (p<0.005). In addition, this combination therapy decreased the extent of demyelination (p<0.05) and increased myelin staining (p<0.05) compared to lesion group. These results indicate that combined vitamin E and D3 therapy improves the myelination status of insults induced by demyelinating toxin ethidium bromide. This effect seems to be mediated by both potentiating the remyelination process and protecting neural cells against apoptotic cell death.

 
Keyword(s): DEMYELINATION, REMYELINATION, ETHIDIUM BROMIDE, CELL DEATH, MYELIN BASIC PROTEIN, VITAMIN E, VITAMIN D3, HIPPOCAMPUS, RAT
 
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