Paper Information

Title: 

EFFECT OF CO-ADMINISTRATION OF ESTROGEN AND PROGESTERONE ON BRAIN IL-1 β AND IL-6 LEVELS AFTER DIFFUSE TRAUMATIC BRAIN INJURY IN FEMALE RAT

Type: SPEECH
Author(s): KHAKSARI MOHAMMAD*,SOLTANI ZAHRA,SHAHROKHI NADER,GHOTBI P.
 
 *NEUROSCIENCE AND PHYSIOLOGY RESEARCH CENTER, KERMAN UNIVERSITY OF MEDICAL SCIENCES, KERMAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

In our previous study, we demonstrated that co-administration of estrogen and progesterone has a neuroprotective effect on brain injury. In the present study, in order to find neuroprotective mechanisms of these steroids, we examined the effect of estrogen and progesterone co-administration on brain IL-1β and IL-6 levels after diffuse traumatic brain injury. Female rats divided into eight groups (1 intact and 7 ovariectomized groups) as follows: 1. Control; 2. Sham; 3. ovariectomized trauma (TBI+OVX); 4. Vehicle; 5. E1+P1; 6. E1+P2; 7. E2+P1; 8. E2+P2. Two weeks after ovariectomy, rats underwent diffuse brain traumatic injury according to Marmarou method. 0.5h after induction of traumatic injury, rats received E1 (33.3μg/kg) or E2 (1mg/kg) together with either P1 (1.7μg/kg) or P2 (8μg/kg) intraperitoneally. Brain amounts of IL-1b and IL-6 were measured 24h after TBI using ELISA. Brain IL-1β level showed a significant increase in TBI group compared to sham and control (p<0.001), while this value significantly decreased in E1+P1 compared to sham, vehicle, E1+P2, E2+P1 and E2+P2 groups (P<0.001). Brain IL-6 levels demonstrated a significant decrease in E1+P2 group compared with vehicle group (P<0.001) and in E2+P2 group compared to vehicle group (P<0.01). Also brain IL-6 levels showed a significant decrease in E1+P2 and E2+P2 groups compared to sham and E1+P1 groups (P<0.001). Co-administration of estrogen and progesterone in physiologic doses decreased brain IL-1b levels. Administration of progesterone with pharmacologic doses in combination decreased brain IL-6 levels. Therefore, exposure to combined female sex hormones may attenuate the production of proinflammatory cytokines early after TBI, and this may be one mechanism by which these sex hormones reduce cerebral edema.

 
Keyword(s): DIFFUSE TRAUMATIC BRAIN INJURY, ESTROGEN, PROGESTERONE, IL-1β, IL-6
 
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