Paper Information

Title: 

EVALUATION OF SPERM SUPEROXIDE ANION PRODUCTION AND APOPTOSIS PROCESS IN EXPERIMENTAL VARICOCELE IN RAT

Type: SPEECH
Author(s): JAFARI ADELEH*,ZAHMATKESH MARYAM,SADEGHIPOUR H.R.9,KAJBAFZADEH ABD ALMOHAMMAD,SARAFNEZHAD A.A.F.,SHAHRESTANI T.
 
 *DEPARTMENT OF PHYSIOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Varicocele is a major cause of male infertility. However, the exact mechanism by which varicocele induces testicular dysfunction is still unknown. Some studies have reported that increased reactive oxygen species (ROS) is a main factor in semen of men with varicocele. The aim of this study was to determine whether intracellular superoxide anion production changes in the sperms of rats with experimental varicocele and to study mitochondrial membrane potential (MMP), Bax and Bcl-2 changes in these rats. Study groups consisted of 23 male rats divided into the following 3 groups: control, sham, and varicocele. Experimental varicocele was established by partial ligation of the left renal vein. Animals were killed 2 months after surgery and dilation of the internal spermatic veins was observed. Then, superoxide anion production, MMP, and Bax and Bcl-2 changes were evaluated. Flow cytometry was used to study sperm superoxide anion production assessed by the dihydroethidium and MMP with rhodamin 123. Bax and Bcl-2 were also assessed by immunohistochemistry. Our results showed that intracellular superoxide anion production was significantly increased and MMP significantly decreased in rats with experimental left varicocele. More Bax-positive cells were seen in the varicocele group. However, there were no distinct changes in Bcl-2 in the varicocele and control groups. These findings suggest that the main source of ROS production in varicocele condition is intracellular, suggesting that elevation of intracellular ROS would be one of the important responsible mechanisms of mitochondrial and tissue damage observed in varicocele.

 
Keyword(s): SUPEROXIDE ANION,VARICOCELE,FLOWCYTOMETRY, APOPTOSIS
 
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