Paper Information

Title: 

APOPTOSIS-RELATED GENE EXPRESSION DURING AGING IN RAT

Type: SPEECH
Author(s): RASHIDI Z.,ZHALEH HOSSEIN*,YOUSEFVAND NADER,YARANI REZA
 
 *DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, RAZI UNIVERSITY, KERMANSHAH, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Apoptosis is a genetically regulated form of cell death that occurs when the cell is exposed to physiological, pathogenic or cytotoxic stimuli. The apoptotic pathways can be initiated by reactive oxygen species (ROS) and inflammatory molecules, both of which are believed to be up-regulated during aging. Rats were divided into two groups, Young group (9 months, n=7) and aged group (24 months, n=7). Animals were sacrificed under deep anesthesia (ketamine, 100 mg/kg, ip) and their brains were dissected out and cleaned of connective tissues. RNA extraction was performed using the RNeasy Plus Mini Kit (QIAGEN) following the manufacturer’s protocol and then c DNA was synthesized. The expression level of genes encoding the anti-apoptotic and pro-apoptotic proteins were compared between young and aged rat brain, using semiquantitative RT-PCR and 1% agarose gel. Results showed that the apoptotic regulator gene P53 and its downstream genes anti-apoptotic Bcl-XL, and pro-apoptotic Bax and Bak were significantly upregulated in aged brain. Pro-apoptotic Bad and Bik, and anti-apoptotic Bcl-2 gene expression levels seemed to show the same trend in both groups and did not significantly change. The results revealed a clear and significant alteration in apoptotic gene expression levels in aged compared to young animals. This fact leads to the hypothesis that apoptosis may be involved in reduced function of brain activity during aging.

 
Keyword(s): APOPTOTIC GENES, AGING, BRAIN, RAT
 
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