Paper Information

Title: 

THREE-DIMENSIONAL CULTURE OF HUVEC CELLS

Type: SPEECH
Author(s): HOZOURI E.*,SEPEHRI HOURI,DELFI L.
 
 *DEPARTMENT OF BIOLOGY, SCHOOL OF SCIENCES, TEHRAN UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Angiogenesis is a highly complex physiological process which is an essential step in tumor proliferation, expansion and metastasis. It has been shown that anti-angiogenic agents help tumor therapy. It has also shown that Pectin and its modified forms can exhibit anti-mutagenic activity and suppress cancer metastasis and proliferation via inhabitation of angiogenesis with no evidence of toxicity or other effects. In the present study three-dimensional culture of HUVEC cells on collagen I to reach capillary tube formation is considered. We used the media of DU-145 cells (prostate cancer cells) which contains vascular endothelial growth factor (VEGF) to promote this process. DU-145 cells were cultured in Ham's F12/DMEM media with 1% FBS while HUVEC cells were maintain in a 20% FBS media. For angiogenesis formation HUVEC cells were grown on collagen I gel for 72h. Then the media of DU-145 cells were placed to their media and the tube formation was followed for a period of 48h. Our results show that however tube structure can form better in the absence of VEGF, the media of DU-145 cells support this formation greatly. In addition this formation can occur only on a three-dimensional culture and VEGF cannot stimulate tube formation in a common culture of HUVEC cells. We will investigate the effect of citrus pectin on angiogenesis by this method in our next research.

 
Keyword(s): ANGIOGENESIS, HUVEC CELLS, DU-145 CELLS, THREE-DIMENSIONAL CULTURE
 
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