Paper Information

Title: 

PRESENCE OF ATP-INSENCETIVE K+ CHANNELS IN ROUGH ENDOPLASMIC RETICULUM (RER) IN RAT HEPATOCYTE

Type: SPEECH
Author(s): SALARI SAJAD*,ELIASI AFSANEH,SAGHIRI R.
 
 *SHAHEED BEHESHTI MEDICAL UNIVERSITY
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Introduction: Very recently, we have suggested the presence of 2 cationic K+ channels in RER. However, one of these channels was reported as an ATP-sensitive K+ channel with 599 pS conductance. Regarding the fact that cationic channels in interacellular organelles as mitochondria and RER play an important role in intracellular signaling and cellular protection, identification of type, kinetic and pharmacological characteristics seems important.
Material & Method: we used single channel recording method after incorporation of microsomes into phosphatydilcholin (PC) bilayer. Subsequently, PC extraction by singletone method, BLM making in 250μm hole by muller technique and RER extraction through Can method were carried out. Channel activity was recorded and data have been interpreted using Noise Free Markov’s analysis and clampfit9 software.
Results: Our results showed a potassium channel with a conductance of ~ 245pS. The channel open probability (Po) was 0.9 and showed a voltage-independent behavior at - 40 to + 40 mV. A selective K+ channel inhibitor, 4-AP (10mM in cytoplasmic face), inhibits the channel activity. However, an intracellular nucleotide, ATP (2.5mM), has no effect on channel function.
Discussion, various potassium channels present in RER which have probably key roles in Ca2+ homeostasis, cell protection and apoptosis.

 
Keyword(s): BILAYER MEMBRANE (BLM) ,ROUGH RETICULUM ENDOPLASMIC (RER) ,PHOSPHATYDILCHOLIN (PC) , 4-AMINOPYRIDINE (4-AP), ATP
 
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