Paper Information

Title: 

SEX-DEPENDENT EXPRESSION OF BETA-ACTIN GENE IN DIFFERENT TISSUES OF RAT

Type: POSTER
Author(s): JORJANI M.,AHANGAR N.A.,SHARIFI KHADIJEH*,SATARIAN L.
 
 *NEUROSCIENCE RESEARCH CENTER, SHAHID BEHESHTI MEDICAL UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Recently it was reported that the expression of commonly used house-keeping genes including Beta–actin are not constant and change in disease states or under the experimental conditions as well as sex-dependent regulation. This phenomenon makes the necessity for validating of any internal control gene between sexes or under the experimental condition. We examined the sex differences in the expression levels of B-actin in different tissues of rat.
Total RNA were extracted from the Brain, Spinal cord, heart, Kidney and liver tissues in male and female rats as well as Uterus in females. Semi quantitative RT-PCR was used to measure the levels of Beta-actin gene in all tissue samples.
Sex-dependent expression of beta actin gene was observed, but it was significant only in brain tissue. Beta actin gene expression in the heart and uterus of females differed significantly with other tissues. Similarly, there was a significant difference in beta actine gene expression between heart and other tissues in male rats. It is suggested to validate the constancy of beta-actin gene under the experimental condition, before using this house-keeping gene as an internal reference marker in molecular studies.

 
Keyword(s): BETA-ACTIN, SEXUAL DIMORPHISM, RAT TISSUES, RT-PCR
 
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