Paper Information

Title: 

BIOCHEMICAL ANALYSIS OF BERBERIS VULGARIS AND ITS EFFECT ON CANCER CELL LINE

Type: SPEECH
Author(s): HANACHI P.*,MOTALEB GH.R.,FAUZIAH O.
 
 *BIOMEDICAL DEPARTMENT, WOMEN RESEARCH CENTER, ALZAHRA UNIVERSITY
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

The Berberis vulgaris (BV) plant has been extensively used as a traditional medicine in some part of Asian region especially in Western Asia. The BV fruits Extracy (BVE) is claimed also to have anti-viral activities, and as a treatment for chronic candidiasis, indigestion and parasites. The present study was conducted to evaluate the biochemical composition and screening of cytotoxic effect of BV on cancer cell line. The amount of nutritional and antinutritional detected in 100g of BV were 11102.81μg±2.01 of Vitamin C, 116.03μg±1.12 of Malic acid and 20.51μg±0.59 of Tannin. Intracellular alkaline phosphatase (ALP) activity is determined by p-nitrophenyl phosphate. The screening of cytotoxic effect was carried out by the microculture tetrazolium salt (MTT) assay on the human liver cancer cell line (HepG2). The BFE with concentration of 5-140 μg/ml was used. The control group cell was without any treatment. The IC50 of BVE was found 106.0±10.1 µg/ml. Beside reduction in cell proliferation the crude extract was capable of enhancing the intracellular protein content in cell cancer line by one fourth while intracellular alkaline phosphatase activity increased by 7 fold. The results showed that processed commercial BV exhibited antioxidant properties, has the ability of reducing cancer cell viability and may have the potential of enhancing the ALP activity probably through structural changes.

 
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