Paper Information

Title: 

EFFECT OF α 3-SUBUNIT SODIUM PUMP HAPPLOINSUFFICIENCY ON SUSCEPTIBILITY TO CORTICAL SPREADING DEPRESSION

Type: SPEECH
Author(s): ZAHEDI KHORASANI M.*,VAKILI A.,JARAHI M.,DREIER J.
 
 *RESEARCH CENTRE AND DEPARTMENT OF PHYSIOLOGY, SEMNAN UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Haploinsufficiency of ATP1A2 encoding the Na+/K+ pump alpha-2 subunit associated with familial hemiplegic migraine type 2(FHM II). Our previous study had shown that a2-Na+ pump happloinsufficiency increased susceptibility to cortical spreading depression (CSD; the likely mechanism for the migraine aura). Regarding similarity between a2 and a3-Na+ pump, we evaluate the role of the a3-Na+ pump happloinsufficiency on susceptibility to CSD in this study.
Methods: We used heterozygote (Het) mice with null mutation in one ATP1A3gene encoding the Na+/K+ pump alpha-3 subunit. Occipital brain slices from wild type (WT) and Het mice were used. CSD was induced by raising [K+] of artificial cerebrospinal fluid (ACSF) in steps of 2.5mM at 30 min intervals. Ion sensitive microelectrode and optical imaging technique were used for recording different factors in
absence and presence of ouabain.
Results: Our results showed there are not any significant difference in CSD threshold and speed of propagation, intrinsic optical signal, DC potential duration and amplitude between Het and WT mice. Ouabain (5μM) increased DC potential-duration and reduced DC- amplitude significantly in both Het and WT mice.
Conclusion: The finding of this study shows that
a3-subunit of Na+ pump insufficiency has not effect on susceptibility to CSD.

 
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