Paper Information

Title: 

ALKALINE PHOSPHATASE AND GLUTATHIONE S-TRANSFERASE ACTIVITY IN HEPATOCARCINOGENESIS INDUCED RATS TREATED WITH BERBERIS VULGARIS EXTRACT

Type: POSTER
Author(s): MOTALEB GH.R.*,HANACHI P.,FAUZIAH O.
 
 *DEPARTMENT OF BIOMEDICAL SCIENCES, FACULTY OF MEDICINE AND HEALTH SCIENCES, UNIVERSITY PUTRA MALAYSIA
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Berberis vulgaris grows in Asia and Europe; the plant is well known in Iran and has been used extensively as a medicinal plant in traditional medicine. Medicinal properties for all parts of the plant which have been reported include tonic, antimicrobial, antiemetic, antipyretic, antipruritic and cholagogue actions, and it has been used in some cases like cholecystitis, cholelithiasis, jaundice, dysentery, leishmaniasis, malaria and gall stones. This study investigated the effect of Berberis. vulgaris fruit extracts (BVE) extraction on the level of glutathione S-transferase (GST) in liver cells cytosol, alkaline phosphatase (ALP) and alpha-feto protein (AFP) in serum of the hepatocarcinogenesis rats. Forty eight Sprague Dawley rats (weighing 150g-250g) were divided into two groups, cancer and non-cancer groups. Each of these groups were divided into four subgroups (n=6) based on three concentrations of extract; 25, 50 and 100 mg/kg. The cancerous group rats were induced hepatocarcinogenesis by diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) and supplemented with 25, 50 and 100 mg/kg B.vulgaris extract for ten weeks. Analysis of variance showed significant difference (p<0.05) in specific activities of ALP and GST between cancer and normal control groups. The finding showed BVE in 25,50 and 100 mg/kg could lower the activity of GST and ALP of rats during the hepatocarcinogenesis. Based on the results obtained BVE extract have the potential in decreasing the severity of hepatocarcinogenesis rats. However, further studies are progress in our laboratory to explore the mode of action of purified component.

 
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