Paper Information

Title: 

THE EFFECT OF DIFFERENT CONCENTRATIONS OF β-GLUCAN ON INTRACELLULAR PROLACTIN SYNTHESIS IN GH3/B6 CELLS

Type: POSTER
Author(s): SHAERZADEH F.*,SEPEHRI H.,HOSSEIN G.,GOLIAEI B.,DELFI L.,RASOULI Y.
 
 *SCHOOL OF BIOLOGY, UNIVERSITY COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Prolactin is a polypeptide hormone of the anterior pituitary gland that was originally named for its ability to promote lactation. Today we know that prolactin has over than 300 different functions in vertebrates include osmoregulation, growth and development, metabolism, behavior, reproduction and immunoregulation. The role of prolactin in immune system is recently investigated. Glucan is a glucose polymer which is the major constituents of the cell wall of fungi, plant and certain bacteria.
Methods: In the present study, we used GH3/B6 cell line which secretes Prolactin and growth hormone. Intracellular Prolactin levels were assayed by ELISA in cells lysets. Cells were treated with 5, 150 and 250μgr/ml concentrations of β-glucan for a period of 48 hours. TRH (50nM) was used as positive control and dextran (50nM), was the negative one.
Result: Our result showed that 150 and 250 μgr/ml concentrations of β-glucan are capable to stimulation Prolactin synthesis significantly from GH3/B6 cells in comparison with control (P<0.05), while 5μgr/ml concentration of β-glucan does not have any significant effect on Prolactin synthesis.
Conclusion: These results show that β-glucan in high concentrations can induce the intracellular synthesis of Prolactin. Since increasing effect of dextran on the Prolactin synthesis have not seen yet so it suggests that this substance acts through its specific receptors.

 
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