Paper Information

Title: 

PHYLOGENETIC RELATIONSHIP OF TWO MULLET SPECIES IN NORTH OF IRAN

Type: POSTER
Author(s): SIADATIAN HAGHIGHI S.S.*,AMINI F.,GHOREYSHI S.A.,HOUSHMAND M.,SALEHI TABAR R.,SHEYBANI Z.
 
 *DEPT. OF MARINE BIOLOGY, SCIENCE AND RESEARCH COMPUS, ISLAMIC AZAD UNIVERSITY
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: The genetic differentiation and phylogenetic relationships between two species of the Mugilidae family (Liza aurata & Liza saliens) were investigated at the mtDNA sequencing on samples taken from Mazandaran (8 individuals) and Gilan (8 individuals) region in southern part of Caspian Sea.
Method: DNA was extracted from muscle tissue, by phenol-chlorophorm method. PCR primers were designed by D-Loop sequence of gene bank.
Results: Using a pair of specific primers a fragment 332bp long was amplified from D-Loop of mtDNA. Products of amplification were recognized by electrophoresis on 1% agarose gel that was stained with ethidium bromide. For sequencing of genomic DNA, PCR products were extracted from agarose gel, Purified with purification kit and sent to the company for sequencing. Analyses of data were done using the software programes and phylogenetic trees were drawn to compare the species.
Conclusion: According to phylogenetic trees, species were clustered in two separate groups, the first one containing Liza aurata, the other Liza saliens.

 
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