Paper Information

Title: 

THE EFFECT OF SEMLIKI FOREST VIRUS (SFV) ON RNAI IN MOSQUITO CELLS

Type: POSTER
Author(s): ATARZADEH YAZDI GH.,FAZAKERLEY J.,FRAGKOUDIS R.,KOHL A.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

RNA silencing is a unique RNA-guided gene regulatory mechanism that operates in a wide range of eukaryotic organisms from plants to mammals. A naturally occurring gene silencing mechanism triggered by double-stranded RNA (dsRNA), RNA interference (RNAi), has recently been described in a number of species including Drosophila. Arboviruses are a group of viruses that usually contain RNA genomes and counteract the innate immune defence mechanisms. Semliki Forest virus (SFV), a mosquito borne virus of the Togaviridae family that can cause encephalitis in vertebrates, encodes a non-structural protein which is antagonistic to the interferon response in mammalian cells but whether SFV can suppress RNAi in mosquito cells is unknown. Then the aim of this project was to investigate whether or not the RNAi activity is present in the U4.4 mosquito cells and also SFV can inhibit the RNAi in these cells. A reporter based assay system was used to measure RNAi activity in U4.4 mosquito cells which transfected with Renilla luciferase reporter gene and Renilla luciferase siRNA. Our data demonstrated that RNAi activity is present in the U4.4 mosquito cells as synthetic siRNAs specifically suppressed Renilla luciferase expression. Furthermore, the results showed that SFV does not interfere with RNAi which established in U4.4 cells but it interrupts the induction of siRNA in U4.4 mosquito cells. Finally, the data confirmed that siRNAs can transfer from one cell to another cell and this suggesting that systemic RNA silencing occurs in U4.4 mosquito cells.

 
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