Paper Information

Title: 

THE EFFECT OF PECTIC ACID ON THE DEATH OF GH3/B6 CELLS

Type: POSTER
Author(s): TATARI F.*,SEPEHRI H.,AZHDARI SOHEYLA,GOLIAEI B.,DELFI L.,RASOULI Y.
 
 *SCHOOL OF BIOLOGY, UNIVERSITY COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Programmed cell death which is called apoptosis is accompanied with special morphological changes. In this study the effects of the pectic acid (derived from apple) on the GH3/B6 cell death is investigated. This substance causes reduction of cancer cell mass while the weight of the mass remains stable.
Methods: GH3/B6 cells were cultured in the Ham’s F12 medium enriched with 15% horse serum and 2.5% fetal bovine serum for 3 days. Then these cells were affected by various amounts of pectic acid (2.5, 50, 100, 150, 200, 250µg/ml) in different periods (24, 48, 72 hour). Bromocriptin (10mM) was utilized as control. Previous studies showed that bromocriptin is one of the apoptotic agents in these cells. The cells viability was detected in the presence of different amounts of pectic acid and bromocriptin by MTT exclusive test. The nuclear changes of cells were explored by propodium iodide (PI) staining. In addition, DNA fragmentation of cells was studied by electrophoresis.
Results: This study has shown that different concentration of pectic acid decreased GH3/B6 cells viability. Furthermore, PI staining showed that DNA was fragmented in the presence of 250μg/ml of pectic acid and this result was confirmed by DNA laddering in electrophoresis.
Conclusion: According to these consequences, we suggest that pectic acid can induce apoptotsis in GH3/B6 cells.

 
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