Paper Information

Title: 

NIGELLA SATIVA SEEDS EXTRACT SUPPRESSES TESTOSTERONE SECRETION FROM MICE LEYDIG CELLS IN VITRO

Type: POSTER
Author(s): ABDUL SAEED S.,ANWAR N.,IQBAL A.,JABEEN Q.,GILANI ANWER
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Nigella Sativa (black seed) is an important medicinal herb. Historically it has been used in many parts of world as a natural remedy for treatment of a wide range of diseases, including many acute as well as chronic conditions. Many studies have explored the pharmacological action (s) of N. Sativa seed extract (NSE) or its active compound (s) on various body systems in vivo or in vitro. However, its role if any, in reproductive system has not been reported yet. In addition, no data is available for the effect of NSE on male reproductive hormonal profile. Most of the available studies have reported its effect on female's reproductive systems but effect of NSE on male reproductive functions remain largely unknown. We designed a study to look at the effect of NSE on testicular steriodogenesis from mice Leydig cells in vitro. Mice testicular cells (80,000/200µl) were incubated in a media containing either no treatment (control) or NSE (1000 to 0.01 µg) or LH 500 µIU alone or in combination with NSE (1000 to 0.01µg). Incubations were carried out for three hours in a shaking water bath at 34°C. Testosterone was measured by radioimmunoassay. At all doses, aqueous NSE significantly inhibited (p<0.05) both basal and LH stimulated in vitro testosterone secretion. At a dose of l000 µg, NSE inhibited 52% of basal testosterone and 97% of LH stimulated testosterone, compared to control (0.32ng/ml ± 0.008) and LH alone (0.33±0.01) respectively. We have demonstrated that both basal and LH stimulated secretion of testosterone from Leydig cells declines significantly in the presence of different doses of crude aqueous NSE in vitro. Further studies are needed to explore the effect of chronic treatment with NSE in male and its potential use as a male contraceptive.

 
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