Paper Information

Title: 

IMPACT OF DURATION AND SEVERITY OF PERSISTENT PAIN ON APOPTOSIS

Type: SPEECH
Author(s): POURAHMAD J.,AHMADIANI A.A.H.,REZAEI MOHSEN
 
 
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

Apoptosis is a highly regulated form of cell death distinguished by the activation of a family of cystein-aspartate proteases (caspases) that cleave various proteins resulting in morphological and biochemical changes characteristic of this form of cell death. Several recent studies have addressed the role of apoptosis in inflammatory and chronic pain states. Caspase-3 plays a central role in mediating nuclear apoptosis including chromatin condensation and DNA fragmentation as well as cell blebbing. The aims of this study were to investigate the effect of duration and severity of persistent pain on induction of apoptosis. Formalin was administered subcutaneously in the Sprague-Dawley rat hind paws 1, 4 or 7 consecutive days, and then the activity of caspase-3 was measured in both rat liver and brain cells. Morphological changes charaterising apoptosis was also studied using Apoptosis Detection kit, Annexin V-Cy3. Our findings showed that caspase-3 activity and apoptotic phenotype significantly increased in brain and liver cells following the increase in duration and severity of formalin induced persistent pain.

 
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