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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   Fall 2004 , Volume 6 , Number 23; Page(s) 144 To 151.
 
Paper: 

ISOLATION AND PURIFICATION OF AG 85 COMPLEX FROM MYCOBADVRIUM BOVIS (BCG) AND ASSESSMENT OF ITS CELL PROLIFERATION RESPONSE IN WHOLE BLOOD

 
 
Author(s):  NIKOUKAR I.*, KAJBAF MJ., MAK VANDI M., FARAJZADEH A., KAMALI E., MOSTAFAEI ALI, HUYGEN K.
 
* Microbiology Dept., Gilan Medical University, Lahijan, Iran
 
Abstract: 
Introduction: Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a major worldwide health problem. The only TB vaccine currently available is anattenuated strain of mycobacterium bovis termed Bacillus Calmette -Guerin (BCG).The efficacy of BCG remains controversial. Mycobacterium secretory proteins are generally considered important antigens for protection against TB. A major protein component of mycobacterial culture filtrate is Antigen 85 complex which is apromising potential vaccine candidate.
Material and Methods: Antigen 85 complex was purified from Mycobacterium bovis (BCG) culture filtrate by sequential chromatography on phenyl Sepharose 4B, DEAE-Sephacel and Superdex G75. Purification was confirmed by SDS-PAGE and immunoblotting with a specific monoclonal antibody. The in vitro ability of Ag 85 complex to stimulate cell proliferation was compared with that of Purified Protein Derivative (PPD) and the polyclonal T cell mitogen PHA in a whole blood assay in which the target cells were derived from 25 healthy PPD-positive and 25 healthy PPD-negative subjects.
Results: Antigen 85 complex was found to have a molecular weight of 30-32 KDa by SDS-polyacrylamide gel electrophoresis and reacted strongly by immunoblotting with the monoclonal antibody specified against Ag 85. The responses to Ag 85 and PPD were significantly higher in cells of PPD- positive than in cells of PPD-negative donors. Eighty eight percent (22/25) of the PPD- positive cells responded to Ag 85 whereas only 16% (4/25) of the PPD-negative cells responded.
Conclusion: The cell proliferation response to Ag 85 complex is significantly different between cells of skin-test positive and skin- test negative subjects and may suggest an immuno-protective role for Ag 85 complex against M. tuberculosis infection.
 
Keyword(s): MYCOBACTERIUM BOVIS (BCG), AG 85 COMPLEX, CELL PROLIFERATION, WHOLE BLOOD ASSAY
 
 
References: 
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Citations: 
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APA: Copy

NIKOUKAR, I., & KAJBAF, M., & MAK VANDI, M., & FARAJZADEH, A., & KAMALI, E., & MOSTAFAEI, A., & HUYGEN, K. (2004). ISOLATION AND PURIFICATION OF AG 85 COMPLEX FROM MYCOBADVRIUM BOVIS (BCG) AND ASSESSMENT OF ITS CELL PROLIFERATION RESPONSE IN WHOLE BLOOD. CELL JOURNAL (YAKHTEH), 6(23), 144-151. https://www.sid.ir/en/journal/ViewPaper.aspx?id=8382



Vancouver: Copy

NIKOUKAR I., KAJBAF M.J., MAK VANDI M., FARAJZADEH A., KAMALI E., MOSTAFAEI ALI, HUYGEN K.. ISOLATION AND PURIFICATION OF AG 85 COMPLEX FROM MYCOBADVRIUM BOVIS (BCG) AND ASSESSMENT OF ITS CELL PROLIFERATION RESPONSE IN WHOLE BLOOD. CELL JOURNAL (YAKHTEH). 2004 [cited 2021June17];6(23):144-151. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=8382



IEEE: Copy

NIKOUKAR, I., KAJBAF, M., MAK VANDI, M., FARAJZADEH, A., KAMALI, E., MOSTAFAEI, A., HUYGEN, K., 2004. ISOLATION AND PURIFICATION OF AG 85 COMPLEX FROM MYCOBADVRIUM BOVIS (BCG) AND ASSESSMENT OF ITS CELL PROLIFERATION RESPONSE IN WHOLE BLOOD. CELL JOURNAL (YAKHTEH), [online] 6(23), pp.144-151. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=8382.



 
 
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