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Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   may 2018 , Volume 16 , Number 5; Page(s) 335 To 340.
 
Paper: 

In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells

 
 
Author(s):  BARATI SHIRIN, MOVAHEDIN MANSOUREH*
 
* Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
Abstract: 
Background: Spermatogonial stem cells are the foundation of spermatogenesis and male fertility. So, their maintenance and culture are very important. Objective: In this study, we assessed protective effects of the Calligonum on in vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial stem cells. Materials and Methods: After 24 hr of culture, the spermatogonial stem cells were treated with 30 μ M dose of H2O2 and then 10 μ g/ml the Calligonum extract was added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain reaction. Results: After 3 wk of treatment, viability in the Calligonum extract+H2O2 group was significantly higher than H2O2 group alone (p=0. 001). In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and P53), was significantly lower than the group treated with H2O2 alone. Conclusion: The results of this study showed that 30 μ M H2O2 increased apoptosis but decreased viability in spermatogonial stem cells. Calligonum has antioxidant properties that can reduce apoptosis, Bax and P53 expression and increase the viability and Bcl-2 expression.
 
Keyword(s): Stem cell,Apoptosis,Calligonum,Viability
 
 
References: 
 
Citations: 
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APA: Copy

BARATI, S., & MOVAHEDIN, M. (2018). In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 16(5), 335-340. https://www.sid.ir/en/journal/ViewPaper.aspx?id=668860



Vancouver: Copy

BARATI SHIRIN, MOVAHEDIN MANSOUREH. In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE). 2018 [cited 2021July27];16(5):335-340. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=668860



IEEE: Copy

BARATI, S., MOVAHEDIN, M., 2018. In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), [online] 16(5), pp.335-340. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=668860.



 
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