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Paper Information

Journal:   JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES)   2018 , Volume 9 , Number 2; Page(s) 147 To 157.
 
Paper: 

Evaluation of Ice Nucleation Activity (INA) and INA Gene Detection in the Bacteria Isolated from Pistachio Trees in Kerman Province, Iran

 
 
Author(s):  Rostami Mahdieh, HASANZADEH NADER*, Khodaygan Pezhman, RIAHI MADVAR ALI
 
* Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Azad University, Tehran, Iran
 
Abstract: 
Ice nucleation active (INA) bacteria are common epiphytic inhabitants that cause frost damage in many plants in the near-zero temperatures. Yet, no studies were found in ice nucleation bacteria associated with pistachio trees. In our earlier study some INA strains were identified and reported. These were assigned as Pseudomonas fragi, P. putida, P. moraviensis and Pantoea agglomerans. In current work, two new strains namely P. viridiflava and Entrobacter cloacea were identified. Their ice nucleation frequency were evaluated and compared with above-mentioned ice positive strains isolated from pistachio trees. Pseudomonas fragi raf3 was considered as the most ice nucleation active bacteria. This was followed by P. putida raf6, P. moraviensis raf1, P. moraviensis raf5, Pantoea agglomeranse raf7, P. viridiflava raf2, Entrobacter cloacea raf8 and Pseudomonas sp. raf4, respectively. To detect INA genes, two sets of degenerate primers were used and partial INA gene sequences were amplified. INA gene sequence )425bp) for Pseudomonas putida raf6, Pantoea agglomerans raf7 and P. fragi raf3 were amplified with primer pair of 3308/3463. Whereas, a fragment of 194bp was detected in Pseudomonas sp. raf4, P. moraviensis raf5 and P. moraviensis raf1 using forward and reverse primer pair of 3076/3463. Entrobacter cloacea raf8 has reported for the first time as epiphytic ice plus strain. The capability of the latter as a bacterial biocontrol agent against insect pests was reported.
 
Keyword(s): Ice nucleation bacteria,Ice nucleation frequency,Kerman Province,Pistachio
 
 
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APA: Copy

Rostami, M., & HASANZADEH, N., & Khodaygan, P., & RIAHI MADVAR, A. (2018). Evaluation of Ice Nucleation Activity (INA) and INA Gene Detection in the Bacteria Isolated from Pistachio Trees in Kerman Province, Iran. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), 9(2), 147-157. https://www.sid.ir/en/journal/ViewPaper.aspx?id=668613



Vancouver: Copy

Rostami Mahdieh, HASANZADEH NADER, Khodaygan Pezhman, RIAHI MADVAR ALI. Evaluation of Ice Nucleation Activity (INA) and INA Gene Detection in the Bacteria Isolated from Pistachio Trees in Kerman Province, Iran. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES). 2018 [cited 2021July27];9(2):147-157. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=668613



IEEE: Copy

Rostami, M., HASANZADEH, N., Khodaygan, P., RIAHI MADVAR, A., 2018. Evaluation of Ice Nucleation Activity (INA) and INA Gene Detection in the Bacteria Isolated from Pistachio Trees in Kerman Province, Iran. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), [online] 9(2), pp.147-157. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=668613.



 
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