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Paper Information

Journal:   JOURNAL OF RESEARCH IN DENTAL SCIENCES   spring 2019 , Volume 16 , Number 1 (59) ; Page(s) 42 To 50.

Identification and sequencing of periodontal-causing bacteria and its Relationship with Interleukin-6 Gene Polymorphism by Tetra-Arms-PCR

Author(s):  Mirzadeh Fahimeh, AMINI KIOMARS*, AMINI PRAVIZ
* Microbiology Dept, School of Basic Sciences, Saveh Azad University, Saveh, Iran
Background & Aim: Periodontal infections are among the most common oral and tooth diseases and recognized as the main cause of the tooth loss after decay. The purpose of this study was to identify and sequencing of the periodontal-causing bacteria and its relation with interleukin-6 gene polymorphism by Tetra-Arms-PCR method. Material and methods: In this case-control study, 100 samples (50 periodontal infections and 50 healthy people) were collected under the supervision of a dentist. After genomic DNA extraction using the sinaclon Company Kit, identification of bacterial strains was performed using 16S rRNA gene amplification. Also, after extraction of cellular DNA from blood samples taken, the polymorphism of the genome encoding IL-6 was performed using the Tetra-Arms-PCR method. The sequencing was carried out by the Sanger method and by Bioneer Company, and finally, the MEGA 7 software was scanned to determine the genetic affinity of the phylogeny tree strains. Results: In the present study, bacteria of Staphylococcus, Lactobacillus, Streptococcus, Acroombocator, Granulicatla Adiasens, Gordonia and Kocuria rhizophila were found in periodontal patients. Also, there was a significant relationship between G/G genotype in-174G/C in periodontal patients compared to control group. (p<0. 05) Conclusions: The correlation between Polymorphism-174G / C in IL-6 and chronic periodontal disease, suggested that, presence of this gene, might be a risk factor for chronic periodontal disease involvement and also, can be an indicator for susceptibility of the mentioned disease involvement.
Keyword(s): Periodontal disease,bacterial sequencing,Interleukin-6,PCR,polymorphism
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