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Paper Information

Journal:   INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY   2018 , Volume 8 , Number 1; Page(s) 975 To 981.

Evaluation of antibiotic resistance pattern in Staphylococcus saprophyticus isolated from patients with urinary tract infection using real-time PCR

Author(s):  Ebrahimi Khorshid, ALIPOUR MAJID*, YAHYAPOUR YOUSEF
* Department of Microbiology, Babol Azad University, Babol, Iran
Staphylococcus saprophyticus is an important agent of urinary tract infections (UTIs), especially among young women. The aim of this study was to identify S. saprophyticus as a common cause of urinary tract infections and determine their antibiotic susceptibility. In this study, 51235 clinical samples were collected from therapeutic centers of Mazandaran. S. saprophyticus was confirmed by the real-time PCR technique through rrs gene and their antibiotic resistance pattern determined by disc diffusion method. Of the 51235 cultivated samples, only 2101 (4. 1%) cases had significant bacteriuria. In this study, the prevalence of S. saprophyticus was 2. 47%. Escherichia coli was the most common bacterium isolated from patients with UTIs at the rate of 61. 06%. S. saprophyticus showed the highest frequency of antibiotic resistance to erythromycin at the rate of 80% and ampicillin and cefotaxime were ranked with the frequency of 55 and 17. 5%, respectively. The present study shows that bacterial resistance is a potential problem in Mazandaran province.
Keyword(s): Urinary Tract Infection,Staphylococcus saprophyticus,Antibiotic Resistance,rrs Gene,PCR
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