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Paper Information

Journal:   INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY   2018 , Volume 8 , Number 1; Page(s) 967 To 974.

Distribution of sul1 gene to sulfonamide resistance in Escherichia coli isolated from Zarjob River (Iran) in Guilan province

Author(s):  ISSAZADEH KHOSRO*, Kezempour Elnaz, Esmaeilieh Vida, SEYEDI SADAF ALSADAT
* Department of Microbiology, College of Basic Sciences, Lahijan Azad University, Lahijan, Iran
Many antibiotics are released into urban wastewater due to incomplete metabolism in humans and ultimately make their path to various natural environments. The use of antibiotics produces a selective pressure that requires antibiotic-resistant bacteria and resistant genes. Sulfonamides are released in aqueous media, the remainder, depending on the chemical activity of the bacteria, is activated against the bacteria and can evolve antibiotic-resistant bacteria (ARB) in the environment Promote natural microbial contamination. The present study evaluates the resistance of E. coli isolated to common antibiotics and especially sulfonamides, and also, since transmission of resistant genes to other bacteria is important in this study, the sul1 genes in E. coli was evaluated. During 5 months (September to December), 25 samples from 5 stations in Zarjob river, were collected. At the first, the MPN test was done, then the samples that were positive, were cultured in EMB and incubated for 24 hours. Antibiogram test was done according to disk diffusion method and CLSI. The serotyping, with multi antiserum kit of pathogenic E. coli was done. For extracting DNA, extraction kit, and PCR test was used to detect the sul1 gene from isolated E. coli. Finally, it was found that 20% of samples were resistance to sulfamethoxazole and all of them were belong to Group III (O128, O125, O44) and according to PCR all of them had sul1 gene. Antibiotic resistance has become a global issue. The most polluted river in the province of Giulan is Zarjob, which can cause pollution of various types of waters, aquatic organisms, fish and other marine organisms used by humans.
Keyword(s): sul1 gene,E.coli,antibiotic resistance,serotyping
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