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Paper Information

Journal:   HORTICULTURAL PLANT NUTRITION   fall 2018-winter 2019 , Volume 1 , Number 2 #R00480; Page(s) 1 To 14.

Effect of silver nanoparticles on germination and growth of two species of thyme in various moisture content

* Department of Range and Watershed, Faculty of natural resources and Geology, University of Kashan
Introduction: Interaction of nutrients and water availability seems to be important environmental factors for plant growth. Furthermore, water stresses one of the most important abiotic factors and limiting germination and early seedling growth and drought stress. Germination is strongly influenced by environmental factors, especially moisture. This study aimed to investigate the effects of silver nanoparticles on germination indices. Thyme is grown in the arid regions; however the effect of moisture content of soil on germination is unclear. On the other hand, it should be possible to increase the germination of thyme by the silver nanoparticles in the soil where the moisture content is not adequate. Materials and Methods: the experiment was conducted in the controlled environment. Two species of thymus (Thymus daenensis Celak and Thymus vulgaris L. ), four moisture content (0,-0. 3,-0. 6 and-0. 9 MPa) and silver nanoparticles (0, 10, 20 and 30 mg/L) were factorial combined in a randomized complete design with four replicates. Seeds were placed in the petri dish sized 8 cm diameter. All seeds and equipment were disinfected to reduced contamination. Poly ethylene glycol (PEG) was used to reduce water potential. For calculation water potential in different concentration of PEG the following formula was used:  s=-(1. 18 × 10-2) C – (1. 18 × 10-4) C2+ (2. 67 × 10-4) CT + (8. 39 × 10-7) C2T where C; concentration, T: temperature. Germination rate and percent were estimated by the following formula, respectively. GR ni t   Results and Discussion: The results showed that germination rate, root and shoot length were significantly affected by both moisture content and Ag concentration. Reduction in moisture content reduced seed germination however with increasing Ag concentration up to 20 mg/L the germination rate was increased. The highest seed germination in all treatments was observed in Thymus vulgaris L. It seems that Ag can be used to improve germination of thyme seed in dry conditions.
Keyword(s): Thyme,seeds,vigor,nanoparticles
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