| Abstract:
Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder featured by insulin resistance and hyperandrogenism. Testosterone enanthate can induce PCOS in mice models. Objective: We investigated the ovary stereological features along with the oxidative stress and inflammatory factors in mice following PCOS induction using testosterone enanthate. Materials and Methods: Twelve female NMRI mice (3 wk old) were divided into 2 groups (n=6/each): Control and PCOS. PCOS was induced through daily injections of testosterone enanthate (1 mg/100g subcutaneous s. c for 5 wk). Finally, ovaries were studied stereologically. The serum levels of the follicle-stimulating hormone, luteinizing hormone, testosterone, interleukin-6, and tumor necrosis factor-α were measured using ELISA kit. Serum levels of Malondialdehyde and the antioxidant capacity were measured relatively using thiobarbituric acid and ferric reducing antioxidant power assay. Results: The mean total volume of ovary and the mean volume of cortex (p<0. 001), volume of oocyte in the preantral (p=0. 011) and antral follicle (p=0. 015), thickness of zona pellucida (p=0. 016), the number of antral follicles (p=0. 012), the serum levels of follicle-stimulating hormone (p<0. 001) and the antioxidant capacity (p=0. 020) reduced significantly in the PCOS group compared to the control. The number of primary (p=0. 017) and preantral (p=0. 006) follicles and the serum levels of testosterone (p<0. 001), Luteinizing hormone (p=0. 002), Malondialdehyde, Interleukin 6 and Tumor necrosis factor-α (p<0. 001) showed a significant increase in the PCOS group compared to the control. Conclusion: Testosterone enanthate induced PCOS causes stereological features in the ovary, increases the oxidative stress and inflammatory markers in mice.
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