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Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   April 2018 , Volume 16 , Number 4; Page(s) 247 To 254.
 
Paper: 

NFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: A case-control study

 
 
Author(s):  TUNCDEMIR MATEM, YENMIS GUVEN, Tombulturk Kubra, ARKAN HULYA, SOYDAS TUGBA, Burak Tek Rasit, Alt?ntas ozlem, OZKARA HAMDI, Kan?gur Sultuybek Gonul*
 
* Department of Medical Biology and, Genetics, Medical Faculty, Istanbul Aydin University, Istanbul, Turkey
 
Abstract: 
Background: A notable proportion of idiopathic male infertility cases is accompanied by oligozoospermia; and yet, the molecular mechanisms of fertilization problem underlying this defect are still unclear. Epithelial cadherin has been involved in several calcium-dependent cell-to-cell adhesion events; however, its participation in gamete interaction has also not been fully investigated. Objective: The aim was to investigate the changes in the expression of E-cadherin, based on the frequency of Single nucleotide polymorphisms in Nuclear Factor Kappa-B 1 and pre-mir-146a in oligospermic men. Materials and Methods: In this case-control study, semen and blood samples of 131 oligospermic men as the case group and 239 fertile healthy men as the control group were analyzed. Variants single nucleotide polymorphisms rs28362491 and rs2910164 were performed using polymerase chain reaction-restriction fragment length polymorphism method and E-cadherin expression were determined by immunoprecipitation studies. Results: ins/ins genotype of rs28362491 was determined as a risk factor for idiopathic oligospermia by 1. 73 times (p=0. 0218), whereas no significant differences were found between the groups concerning pre-mir-146a rs2910164 polymorphism (p=0. 2274 in case of GC genotype and p=0. 9052 in case of GG genotype). Combined genotype analysis results did not show any notable differences between the multiple comparisons of 28362491-rs2910164 in oligospermic men and control groups. In addition, E-cadherin expression of oligospermic men with ins/ins genotype was significantly lower than patients with del/ins genotype (p=0. 0221). Ecadherin expression level was low in oligospermic men with respect to the control group in presence of ins/ins genotype of NFKB1 gene. Conclusion: These results suggest that ins allele prevents binding of surface proteins to spermatozoa, leading to a low affinity of sperm-oocyte interaction in oligospermic men.
 
Keyword(s): E-Cadherin,Oligospermia,Male infertility,Nuclear factor kappa B,Mir-146a
 
References: 
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