Paper Information

Journal:   JOURNAL OF MICROBIAL WORLD   January 2019 , Volume 11 , Number 4 (37) #l00574; Page(s) 392 To 403.
 
Paper: 

Identification of resistant eggplant cultivars to Fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt using molecular markers in Iran

 
 
Author(s):  Safikhani Negin*, MORID BAHAR, ZAMANIZADEH HAMIDREZA, HAJMANSOOR SHAHAB
 
* Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Azad university, Tehran, Iran
 
Abstract: 
Background & Objectives: Eggplant fusarium wiltis an important factor of yield reduction throughout the world. The ability of this pathogen to survive for several consecutive years within the soil, even in the absence of the host, has made it difficult to control. Producing and using the resistant cultivar is the most effective and suitable method to control this disease. This study was aimed to identify fusarium wilt resistant eggplant cultivars. Materials & Methods: First, leaf samples of domestic and hybrid eggplant cultivars were gathered from 28 provinces in Iran and then DNA extraction from young leaves of the cultivars was carried out using CTAB method. Four markers including CAPS, RAPD, SRAP, and SCAR were used to determine the resistant cultivars. In order to confirm the results, resistance and sensitivity of the genotypes were assessed in greenhouse conditions, as well. Results: Out of 20 genotypes of this study, 13 showed index resistance band using CAPS, RAPD, and SRAP of molecular markers. On the other hand, the SCAR marker could not separate the resistant cultivars from the sensitive ones. Phenotype assessment of native and hybrids resistant cultivars in greenhouse condition confirmed the results of the molecular analysis. Conclusion: In general, the use of resistant cultivars obtained in this study using molecular markers is recommended for planting in areas with fusarium wilt disease.
 
Keyword(s): Fusarium wilt,Eggplant,Molecular markers,Resistance gene
 
References: 
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