Paper Information

Journal:   PROGRESS IN BIOMATERIALS   2019 , Volume 8 , Number 1; Page(s) 31 To 42.
 
Paper: 

A silk fibroin/decellularized extract of Wharton’ s jelly hydrogel intended for cartilage tissue engineering

 
 
Author(s):  Basiri Arefeh, FAROKHI MEHDI, Azami Mahmoud, EBRAHIMI BAROUGH SOMAYEH, MOHAMADNIA ABDOLREZA, RASHTBAR MORTEZA, Hasanzadeh Elham, BAGHABAN ESLAMINEJAD MOHAMADREZA, AI JAFAR*
 
* Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
 
Abstract: 
A hybrid hydrogel was obtained from decellularized extract from Wharton’ s jelly (DEWJ) and silk fibroin (SF) and characterized for cartilage tissue engineering. Wharton’ s jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton’ s jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β 1 (TGF-β 1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for cartilage tissue engineering applications.
 
Keyword(s): Decellularization ·,Wharton’,s jelly ·,Silk fibroin ·,Hydrogel ·,Cartilage tissue engineering
 
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