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Paper Information

Journal:   PROGRESS IN BIOMATERIALS   2019 , Volume 8 , Number 1; Page(s) 43 To 50.

A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans

Author(s):  Asadi Shahi Sabrieh*, ROUDBAR MOHAMMADI SHAHLA*, ROUDBARY MARYAM, Delavari Hamid
* Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Candida albicans (C. albicans) belongs to the opportunistic fungal pathogens, which cause a wide spectrum of infections in immune-compromised patients. Graphene oxide (GO), a biocompatibility agent, has been reported to exhibit effective antimicrobial activity. In the present study, a graphene oxide/fluconazole (GO/Flu) compound was synthesized and characterized using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The antifungal activity of GO/Flu was examined against fluconazole-resistant C. albicans (ATCC 10231) compared to GO and Flu using the broth microdilution method, according to CLSI protocol. DNA fragmentation was assessed through the antifungal mechanism of GO/Flu. The release of Fluin PBS medium was measured. Moreover, the cytotoxicity effect of GO/Flu on SW480 cell line was evaluated. Indeed, adhesion ability of C. albicans-treated GO/Flu against SW480 cell line was assessed. The minimum inhibitory concentration (MIC) of GO, Flu, and GO/Flu was determined at 800 μ g/mL, 16 μ g/mL, and 400– 9 μ g/mL, respectively. Notably, GO/Flu exhibited an intense antifungal activity compared to GO and Flu. In addition, GO/Flu showed much less cell toxicity against SW480 cell line than GO and Flu (P < 0. 05). The release determination of Flu in PBS (pH 7. 4) medium was 72. 42%. GO/Flu reduced the adhesion ability of C. albicans to SW480 cell line significantly. DNA fragmentation assay indicated that GO/Flu potentially degraded the DNA of C. albicans and caused a fungicidal influence. According to the findings, GO/Flu could enhance the antifungal activity against C. albicans through DNA fragmentation with low cytotoxicity effect.
Keyword(s): Antifungal agent ·,Candida albicans ·,Graphene oxide ·,Fluconazole
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