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Paper Information

Journal:   JOURNAL OF MICROBIAL WORLD   September 2018 , Volume 11 , Number 3 (36) #R00473; Page(s) 278 To 287.

The effect of probiotic lactobacilli on the attachment power and biofilm formation of Escherichia coli isolated from urinary tract infections

Author(s):  Bandari Soudeh, Arbab Soleimani Nazila*, TAJBAKHSH ELAHEH
Background & Objectives: Scientists are of the conviction that probiotic bacteria can ameliorate urinary tract infection by inhibiting colonization, attachment, and growth of uropathogenic Escherichia coli. The aim of this research was to investigate the anti-adhesive effect of two probiotic Lactobacilli including Lactobacillus plantarum and Lactobacillus casei on uropathogenic E. coli (UPEC). Materials & Methods: In research in 1395, 35 urinary tract infection (UTI) samples were collected from one hospital in Tehran and based on biochemical analysis 27 UPEC samples were identified. The ability of biofilm-formation and the presence of genes (papG and fimH) involved in biofilmformation was investigated by microtiter plate and PCR methods, respectively. Co-aggregation and anti-adhesive effects of two probiotic lactobacilli including L. plantarum and L. casei against UPEC were studied by co-aggregation and microtiter plate method, respectively. Results: Among 27 isolates, 77%, 15%, and 5% showed strong, mediate and the weak ability of biofilm-formation, respectively, and 3% had no ability. Among 15 UPEC which had a strong biofilm-formation ability, 13 (86%) and 15 (100%) had papG and FimH genes, respectively. The average of co-aggregation between L. planetarium and L. casei with UPEC was gained 49.13% and 46.25%, respectively. The mean anti-adhesive effect of L. plantarium and L. casei against pathogenic bacterium was 62% and 58%, respectively. Conclusion: Further studies on the anti-adhesive effect of probiotic lactobacilli are suggested to prevent UPEC prevalence.
Keyword(s): Escherichia coli,Urinary infection,Anti-adhesive effect,Probiotic Lactobacilli
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