Paper Information

Journal:   BASIC AND CLINICAL NEUROSCIENCE   May-June 2019 , Volume 10 , Number 3; Page(s) 225 To 234.
 
Paper: 

Sex Differences and Role of Gonadal Hormones on Glutamate Level After Spinal Cord Injury in Rats: A Microdialysis Study

 
 
Author(s):  Samandari Razieh, HASSANPOUR EZATTI MAJID, FAKHRI SAJAD, ABBASZADEH FATEMEH, JORJANI MASOUMEH*
 
* Neuroscience Research Center, Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
 
Abstract: 
Introduction: Sex differences in outcomes of Spinal Cord Injury (SCI) suggest a sexhormone-mediated effect on post-SCI pathological events, including glutamate excitotoxicity. This study aimed to investigate the importance of gonadal hormones on glutamate release subsequent to SCI in rats. Methods: After laminectomy at T8-T9, an electrolytic lesion was applied to the spinothalamic tracts of male and female rats. Using spinal microdialysis, we assessed glutamate levels at the site of lesion in both intact and gonadectomized rats for 4 hours. In this way, we examined the sex differences in the glutamate concentrations. Results: The peak retention time of glutamate level was 10. 6 min and spinal glutamate concentration reached a maximum level 40 min following SCI. In male SCI rats, gonadectomy caused a significant elevation of glutamate level (P<0. 001) following injury which was maximum 40 min post-SCI as well. However, no significant alterations were seen in gonadectomized female rats. Conclusion: The significant differences in glutamate levels between both intact and gonadectomized SCI male and female rats show the sex-hormone-related mechanisms underlying the molecular events in the second phase of SCI. It seems that the role of male gonadal hormones to prevent glutamate excitotoxicity is more prominent. The exact mechanisms of these hormones on the functional recovery after SCI should be clarified in further studies.
 
Keyword(s): Spinal Cord Injury,Microdialysis,Glutamate,Sex hormones
 
References: 
 
  pdf-File tarjomyar Yearly Visit 38
 
Latest on Blog
Enter SID Blog