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Paper Information

Journal:   MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)   2018 , Volume 32 , Number 1; Page(s) 0 To 0.

Resveratrol suppresses hyperglycemia-induced activation of NF-κ B and AP-1 via c-Jun and RelA gene regulation

Author(s):  Amiri Fatemeh sadat, SABOOR YARAGHI ALI AKBAR, KOOHDANI FARIBA, Norouzzadeh Marjan, Sharifi Loghman, Seyyed Salehi Monireh Sadat, EBRAHIMI AMIRPASHA
Background: Resveratrol (RSV) provides several important biological functions in wide variety of cells. In this study, we investigated the molecular mechanisms underlying anti-inflammatory effect of RSV on HepG2 cells by assessing the gene expression of RelA and c-Jun-subunits of NF-κ B and AP-1 transcription factors. Methods: HepG2 cells were settled in a serum-free medium with high concentrations of glucose (30 mM) and insulin (1 μ M) overnight and were then incubated with RSV (5, 10, and 20 μ M) for 24 and 48 hours. Real time quantitative polymerase chain reaction (qRT-PCR) was used to determine RelA and c-Jun expression. Results: RSV diminished hyperglycemia/hyperinsulinemia stimulated expression of c-Jun dose-dependently after 24 and 48 hours (p<0. 05). In addition, RelA gene expression was decreased dose-dependently in all RSV doses after 48-hour incubation (p<0. 05). Our results indicated that RSV may reduce NF-κ B and AP-1 activity via RelA and c-Jun gene regulation. Conclusion: The findings of the present study demonstrated that RSV may be considered as a preventative and therapeutic agent for antagonizing inflammation in Hepatocellular carcinoma (HCC).
Keyword(s): AP-1,c-Jun,Hyperglycemia,Hyperinsulinemia,Inflammation,Resveratrol,NF kappa B,MAPK
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