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Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   DECEMBER 2017 , Volume 15 , Number 12; Page(s) 779 To 786.
 
Paper: 

Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study

 
 
Author(s):  Zare Zohreh, ABOUHAMZEH BEHESHTEH, MASTERI FARAHANI REZA, SALEHI MOHAMMAD, MOHAMMADI MOSLEM*
 
* Department of Physiology and Pharmacology, Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Kilometer 18 of Khazarabad Road, Khazar Sq., Sari, Iran. Postal Code: 4847191971
 
Abstract: 
Background: Oocyte developmental competence is one of the key factors for determining the success rate of assisted reproductive technique. Objective: The aim of the current study was to investigate the effect of L-carnitine (LC) supplementation during in vitro maturation (IVM), on preimplantation embryo development and expression of genes involved in embryo competence derived from oocytes selected with brilliant cresyl blue (BCB) test. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from NMRI mice ovaries. COCs were stained with BCB and then BCB+ (colored cytoplasm) oocytes cultured in IVM medium supplemented with 0. 3 or 0. 6 mg/ml LC. COCs untreated with LC were used as control. Fertilization rate and blastocyst development rate were determined after in vitro fertilization. In addition, quantitative reverse transcriptase polymerase chain reaction was used to measure relative genes expression related with development (Ccnb1, Mos, Ces5, and Dppa2) and apoptosis (Bax and Bcl-xL) in oocytes and embryos. Results: Oocytes treated with both LC concentrations showed higher blastocyst development rate compared with untreated oocytes (p<0. 01). Moreover, fertilization rate was increased in oocytes treated with 0. 6 mg/ml LC (p<0. 01). Treatment of oocytes with both LC concentrations increased (p<0. 01) the level of Ccnb1 mRNA in MII oocytes. The two-cell stage embryos and blastocysts derived from LC-treated oocytes (0. 6 mg/ml) showed increased the expression levels of Dppa2 and Bcl-xl mRNA, respectively (p<0. 01). Conclusion: The results of the present study show that adding of LC to the IVM medium of BCB+ oocytes can ameliorate reproductive success following in vitro fertilization.
 
Keyword(s): L-carnitine,In vitro fertilization,Oocyte,Gene expression
 
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