Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   OCTOBER 2017 , Volume 15 , Number 10; Page(s) 641 To 648.
 
Paper: 

Thymoquinone as a natural spermostatic substance in reproductive medicine: An experimental study

 
 
Author(s):  GOLSHAN IRANPOUR FARHAD, Fazelian Khatereh, DASHTI GHOLAM REZA*
 
* Department of Anatomical Sciences, Isfahan University of Medical Sciences, Hezarjarib St., Isfahan, Iran
 
Abstract: 
Background: Nonoxynol-9 a nonionic surfactant is widely used for its spermicidal effects. Finding new sperm immobilizing agents is necessary because Nonoxynol-9 damages the tissues of female reproductive system. Objective: The aim of this study was to evaluate the effects of Thymoquinone (TQ) as a potential spermostatic compound on the motility and viability of human spermatozoa. Materials and Methods: In this experimental study, the effects of 5, 10, 20, 50, 100 μ g/ml, 1 and 10 mg/ml of TQ on normozoospermic semen samples were investigated. Sperm motility and viability were compared between untreated and TQ-treated aliquots of each semen sample. To evaluate the effects of TQ on the alteration of mitochondrial membrane potential (MMP), 32 semen samples were examined using 50 μ g/ml of TQ. Flow cytometric analysis was performed after staining of spermatozoa with JC-1. Results: Doses above 20 μ g/ml of TQ could eventually immobilize all spermatozoa in culture medium. Adding 50 μ g/ml of TQ did not significantly diminish the percentage of viable spermatozoa and flow cytometry results revealed that this amount of TQ could decrease sperm MMP. Conclusion: TQ could discontinue the movement of sperm cells in medium without reducing the population of live spermatozoa. It is more likely that TQ exerts its spermostatic action by mitigating the MMP of spermatozoa. Therefore, TQ could be considered as a potential new natural spermostatic chemical.
 
Keyword(s): Nigella sativa,Sperm immobilizing agents,Sperm motility,Thymoquinone
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 63
 
Latest on Blog
Enter SID Blog